Minji Kim (동의대학교)

Hyeyoung Lee (Department of Applied Chemistry·Food Science and Technology, Dong-eui University, 176 Eomgwang-ro, Busanjin-gu, Busan 47340, Korea)

Minji Kim (동의대학교)

Jang-Eun Lee (Department of Applied Chemistry·Food Science and Technology, Dong-eui University, 176 Eomgwang-ro, Busanjin-gu, Busan 47340, Korea)

Eunseon Ryu (Food Science & Technology Major, Division of Applied Bioengineering, Dong-eui University, Korea)

Seojin Choi (Food Science & Technology Major, Division of Applied Bioengineering, Dong-eui University, Korea)

Hyeyoung Lee (Department of Applied Chemistry·Food Science and Technology, Dong-eui University, 176 Eomgwang-ro, Busanjin-gu, Busan 47340, Korea)

Accurate profiling and quantitation of intracellular sugars and polar metabolites are essential for understanding yeast central carbon metabolism and optimizing precision fermentation. In this study, we established an integrated LC-MS platform combining untargeted and targeted approaches for Saccharomyces cerevisiae. Polar metabolites were separated using a HILIC column and analyzed by untargeted Q-TOF MS, enabling detection of a broad range of metabolites, including sugars, sugar phosphates, TCA cycle intermediates, glycerol metabolites, and amino acids.

Various extraction solvents were evaluated, and acetonitrile:water showed the highest extraction efficiency. Based on this, a targeted LC-MS/MS method using a triple quadrupole system was developed for qua​ntitative analysis. In addition, a unified LC-MS/MS method enabling simultaneous analysis of sugars and polar metabolites was established.

This platform enables quantitative metabolomics and monitoring of intracellular metabolic states and may support metabolic pathway optimization and microbial cell factory development for precision fermentation.