2026년
한국질량분석학회 여름학술대회 및 총회
2026. 08.19 (수) ~ 2026. 08.21 (금)
창원컨벤션센터(CECO)
초록검색
| 제목 | Development of a selective liquid culture method and sample-preparation workflow for rapid carbapenemase identification using MALDI-TOF mass spectrometry |
|---|---|
| 작성자 | 황서현 (씨젠의료재단) |
| 발표구분 | 포스터발표 |
| 발표분야 | 4. Medical / Pharmaceutical Science |
| 발표자 |
황서현 (씨젠의료재단 질량분석연구소 ) |
| 주저자 | 황서현 (씨젠의료재단 질량분석연구소 ) |
| 교신저자 | |
| 저자 |
황서현 (씨젠의료재단 질량분석연구소 ) |
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Rapid detection of carbapenemase-producing Enterobacterales (CPE) is essential for infection control. Although matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) enables rapid bacterial identification, single-day detection of CPE directly obtained from rectal swab specimens remains challenging, because of low target abundance and competing non-CPEmicroorganisms. Therefore, we developed a selective liquid culture method and sample-preparation workflow to improve target detection. Multiple conditions such as temperature, antibiotics, oxygen availability, incubation time, and pretreatment were evaluated. The optimized workflow consisted of 4-h culture in artificial anaerobic condition using the antibiotics combination, followed by sample pretreatment and MALDI-TOF MS analysis. The resulting spectra were interpreted using the in-house CPE Typer program, which detects predefined target protein mass and assigns carbapenemase types. Preliminary evaluation using 217 clinical specimens showed high accuracy (90%). These findings suggest that our selective liquid culture combined with the CPE Typer program may support rapid carbapenemase typing from clinical specimens using MALDI-TOF MS. Larger independent cohorts are required to confirm typing performance and prediction accuracy of bacterial species |
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