태규리 (한국표준과학연구원, 과학기술연합대학원대학교)

최기환 (한국표준과학연구원, 충남대학교 분석과학기술대학원)

김병주 (한국표준과학연구원, 과학기술연합대학원대학교)

태규리 (한국표준과학연구원, 과학기술연합대학원대학교)

이홍희 (한국표준과학연구원)

최기환 (한국표준과학연구원, 충남대학교 분석과학기술대학원)

김병주 (한국표준과학연구원, 과학기술연합대학원대학교)

Free pantothenic acid in candidate certified reference material was accurately measured with the high-order method based on isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS). After extraction with 10 mM ammonium acetate (pH 5) and clean-up using protein precipitation, the sample was analyzed using LC-MS. Commercially available beef meat was freeze-dried, pulverized, sieved, and homogenized for the production of the candidate reference material. Then, the processed material was bottled in 15 g portion. The prepared candidate reference material was sufficiently homogeneous as among-bottle relative standard deviation was 0.64% for pantothenic acid. The stability of pantothenic acid was evaluated under -70 ℃, -20 ℃, 4 ℃, and room temperature for up to 12 months, 6 months, 1 months, and 2 weeks, respectively. The content of pantothenic acid was stable throughout each condition. The concentration of free pantothenic acid in the candidate certified reference material​ was 13.15 ± 0.34 mg/kg.

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