2024년
2024 한국질량분석학회 여름학술대회 및 총회
2024. 08.28 (수) ~ 2024. 08.30 (금)
군산새만금컨벤션센터(GSCO)
초록검색
| | 2024 한국질량분석학회 여름학술대회 및 총회 Brief Oral Presentaionof Selected Posters | |
| 제목 | Enhanced LC/MRM-MS Platform for Sensitive Detection of Cyclic Imines in Marine Mollusks |
|---|---|
| 작성자 | 유재영 (충남대학교 분석과학기술대학원) |
| 발표구분 | 포스터발표 |
| 발표분야 | 3. Food & Environment |
| 발표자 |
Jae Young Yu (Graduate School of Analytical Science and Technology, Chungnam National University) |
| 주저자 | Jae Young Yu (Graduate School of Analytical Science and Technology, Chungnam National University) |
| 교신저자 |
Hyun Joo An (Graduate School of Analytical Science and Technology, Chungnam National University) |
| 저자 |
Jae Young Yu (Graduate School of Analytical Science and Technology, Chungnam National University) Hye Won Kim (Graduate School of Analytical Science and Technology, Chungnam National University) Nari Seo (Graduate School of Analytical Science and Technology, Chungnam National University) Young Sang Kim (Department of Marine Life Sciences, Jeju National University) You Jin Jeon (Department of Marine Life Sciences, Jeju National University) Hyun Joo An (Graduate School of Analytical Science and Technology, Chungnam National University) |
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Rapid changes in the marine ecosystems have led to an increased exposure of a novel marine biotoxin group known as cyclic imines (CIs). To mitigate associated risks, sensitive monitoring platforms for detecting CIs in marine organisms are essential. CIs analysis using LC-MS presents analytical challenges due to the presence of high levels of analogues and interference from lipophilic substances. Therefore, an improved method that can accurately and rapidly detect various types of CIs while minimizing interference effects is needed. In this study, we optimized purification conditions and monitored seven CIs (PnTX E, PnTX F, PnTX G, 13-des-SPX C, 13,19-dides-SPX C, 20-des-SPX G, and GYM A) simultaneously using LC/MRM-MS-based quantitative platform. Initially, we spiked seven CIs into a shellfish matrix and assessed the efficiency of purification methods such as membrane filter and SPE. The HLB-SPE with methanol condition provided optimal results across sensitivity, recovery, and reproducibility. Notably, recovery rates were stable, ranging from 80 to 120% across a quantitative linear range. Using this platform, we successfully detected CIs in marine mollusks collected from the Korean Peninsula with ppt-level. Ultimately, our platform provides a valuable reference for enhancing quantitative accuracy of LC/MS-based CI monitoring.
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