이선호 (한국기초과학지원연구원)

황금숙 (한국기초과학지원연구원)

이선호 (한국기초과학지원연구원)

정영애 (한국기초과학지원연구원)

한기훈 (고려대학교)

황금숙 (한국기초과학지원연구원)

Recent research highlights the importance of lipid metabolism in various brain diseases such as Alzheimer's disease, Parkinson's disease, stroke, and epilepsy. The brain is the second highest lipid-containing organ in the body, and lipid metabolism within the brain plays a crucial role in numerous physiological activities and acts as signaling molecules. The brain consists of various regions with distinct functions, and the types of cells that constitute these regions vary, leading to differences in lipid composition. Therefore, studying lipid metabolism in the brain is essential due to its central role in brain health and activity. In this study, we used Liquid Chromatography (LC)-Trapped Ion Mobility Spectrometry (TIMS)-Time of Flight (TOF) to analyze the lipid profiles in the brain tissues of 28-week-old mice. We aimed to compare the lipid composition of the cerebral cortex, responsible for essential functions such as sensation, movement, and language, with that of the hippocampus, which is responsible for long-term memory and learning. We identified a total of 374 lipid species, classified into 25 classes within five categories: Glycerolipids, Glycerophospholipids, Fatty Acyls, Sphingolipids, and Sterol Lipids. Although there were no differences in the higher lipid categories or classes between the cerebral cortex and hippocampus, we observed the differences in specific lipid classes within each class. Notably, Phosphatidylcholine (PC), Phosphatidylethanolamine (PE), and Diacylglycerol (DG) were more abundant in the cerebral cortex, while Sphingomyelin (SM), Cholesteryl Ester (CE), and Triacylglycerol (TG) were more abundant in the hippocampus. This study highlights the differences in lipid composition between the cerebral cortex and hippocampus, which are responsible for major brain functions. It contributes to our understanding of brain function and disease, supporting the critical role of lipid metabolism in brain health.​