2024년
2024 한국질량분석학회 여름학술대회 및 총회
2024. 08.28 (수) ~ 2024. 08.30 (금)
군산새만금컨벤션센터(GSCO)
초록검색
| 제목 | Bioactivities and in silico prediction of quinic acids from Vitex rotundifolia obtained by supercritical fluid extraction |
|---|---|
| 작성자 | 하인진 (경희의료원) |
| 발표구분 | 포스터발표 |
| 발표분야 | 4. Medical / Pharmaceutical Science |
| 발표자 |
In Jin Ha (Korean Medicine Clinical Trial Center (K-CTC), Korean Medicine Hospital, Kyung Hee University) |
| 주저자 | Soojung Yu (Department of Natural Cosmetics Science, Graduate School, Sunchon National University) |
| 교신저자 |
Mina Lee (College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University) |
| 저자 |
Soojung Yu (Department of Natural Cosmetics Science, Graduate School, Sunchon National University) Duc Dat Le (College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University) Yeong Su Jang (Nano Bio Research Center, Jeonnam Bio Foundation) Vinhquang Truong (Department of Natural Cosmetics Science, Graduate School, Sunchon National University) In Jin Ha (Korean Medicine Clinical Trial Center (K-CTC), Korean Medicine Hospital, Kyung Hee University) Mina Lee (College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University) |
|
Molecular network and mass dereplication guided isolation of acyl-quinic acids (AQAs), two novel (5 and 7) and eight known compounds, from V. rotundifolia. Their structures were identified by using multiple spectroscopic methods, reaction mixtures, and modified Mosher and PGME techniques. These compounds were evaluated for their anti-inflammatory and antioxidant properties. Notably, compounds 1, 3, 4, 6, 8, and 9 exhibited significant DPPH radical scavenging activity. In LPS-induced HT-29 cells, compounds 2-7 significantly inhibited IL-8 production. Furthermore, compounds 3-5 and 7 markedly suppressed NO production, while compounds 1-10 effectively inhibited IL-6 production in LPS-induced RAW264.7 cells. Western blot analyses revealed that compounds 3-5 and 7 reduced iNOS and COX-2 expression, and compounds 2-5, 7, and 8 also diminished the expression levels of p38 MAPK phosphorylation. Molecular docking studies demonstrated that the active compounds have a high binding affinity with the IL-8, iNOS, COX-2, and p38 MAPK proteins through interactions with essential amino acids within the binding pockets of these complexes. These findings suggest that compounds 3-5 and 7 hold promise as potential therapeutic agents for the treatment of inflammatory diseases. |
|