여명 (한국기초과학지원연구원)

여명 (한국기초과학지원연구원)

여명 (한국기초과학지원연구원)

김진영 (한국기초과학지원연구원)

안다솜 (한국기초과학지원연구원)

황희연 (한국기초과학지원연구원)

김혜진 (KAIST)

김필남 (KAIST)

The development of the PTMOmics pipeline aims to better understand post-translational modifications (PTMs), which are crucial regulators of protein function. PTMs contribute significantly to protein functional diversity, but PTM proteomics often yields less information compared to global proteomics due to the substoichiometric abundance of PTM proteins. To address this challenge, we developed the PTMOmics pipeline, which integrates global proteomics with PTM proteomics. First, GO network analysis is performed on proteins identified through global proteome analysis. Next, proteins within each GO network are listed and processed through the PTMOmics pipeline, which automatically collects PTM information from open-access protein databases such as UniProt. The pipeline then visualizes the distribution of major PTMs within a GO network and the modified sequence positions of each protein. We applied this approach to investigate PTM-associated relationships and their roles in GO networks identified through global proteomics in colon cancer studies. Global proteomics revealed changes in proteins involved in ECM-related pathways, highlighting glycosylation as a key PTM. We discovered that protein glycosylation patterns were altered in colon cancer.