2025년
한국질량분석학회 여름학술대회 및 총회
2025. 08.27 (수) ~ 2025. 08.29 (금)
부산항국제전시컨벤션센터(BPEX)
초록검색
| | 한국질량분석학회 여름학술대회 및 총회 Brief Oral Presentaionof Selected Posters | |
| 제목 | Development of a Sensitive LC-MS/MS Method and Its Application to the pharmacokinetic of Inotoidol in Mouse Plasma |
|---|---|
| 작성자 | 이소현 (UST) |
| 발표구분 | 포스터발표 |
| 발표분야 | 4. Medical / Pharmaceutical Science |
| 발표자 |
이소현 (UST, 한국생명공학연구원) |
| 주저자 | 이소현 (UST, 한국생명공학연구원) |
| 교신저자 |
이경륜 (UST, 한국생명공학연구원) |
| 저자 |
이소현 (UST, 한국생명공학연구원) 김민주 (한국생명공학연구원) 조인경 (한국생명공학연구원) 정현철 (한국생명공학연구원) 이경륜 (UST, 한국생명공학연구원) |
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Inotodiol, a lanostane-type triterpenoid derived from Inonotus obliquus (Chaga mushroom), has shown anti-inflammatory, antioxidant, and anticancer activities. Despite its biological potential, pharmacokinetic data on inotodiol remain limited. This study aimed to characterize its pharmacokinetic profile in mouse plasma following oral and intravenous administration. A sensitive and selective LC-MS/MS method was developed using protein precipitation and chromatographic separation on a Shimadzu Nexera UPLC coupled with a SCIEX QTRAP 6500 operating in APCI mode. Interestingly, lower source temperatures improved signal intensity, contrary to typical APCI conditions requiring higher heat. Optimal parameters included a 250 °C source temperature, curtain gas of 35 psi, and collision energy of 30 V. Inotodiol was quantified by MRM (m/z 425.265 → 247.000), showing excellent linearity (r² > 0.998). Pharmacokinetic analysis revealed rapid clearance after intravenous injection and low oral bioavailability. After oral dosing, Cmax was 548.45 ng/mL at 2.00 h, with AUCinf of 2068.49 ng·h/mL. The absolute bioavailability (F = 0.14) suggests significant first-pass metabolism or poor intestinal absorption. These findings highlight the need for improved delivery strategies to enhance systemic exposure. This work provides foundational pharmacokinetic data to support future formulation and preclinical studies of inotodiol. |
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