Yun Ji Kang (Cosmetic Research Division, National Institute of Food and Drug Safety Evaluation)

Young Me Song (Cosmetic Research Division, National Institute of Food and Drug Safety Evaluation)

Yun Ji Kang (Cosmetic Research Division, National Institute of Food and Drug Safety Evaluation)

Euijung Kim (Cosmetic Research Division, National Institute of Food and Drug Safety Evaluation)

Su-Jin Kim (Cosmetic Research Division, National Institute of Food and Drug Safety Evaluation)

Ji Hye Han (Cosmetic Research Division, National Institute of Food and Drug Safety Evaluation)

Taehyung Yoon (Cosmetic Research Division, National Institute of Food and Drug Safety Evaluation)

Young Me Song (Cosmetic Research Division, National Institute of Food and Drug Safety Evaluation)

 Triclosan(TCS) is widely used as an active ingredient in numerous household and personal care products, including shampoos, soaps, creams, mouthwashes and toothpastes, due to its antimicrobial efficacy. According to domestic cosmetic regulations, TCS is permitted at concentrations of up to 0.3% as preservative in rinse-off cleansing products, deodorants (excluding spray products), face powder, and foundations intended to conceal skin imperfections (e.g. blemish concealer); and it is prohibited to use in other products. When used for purposes other than preservation, it is permitted at concentrations of up to 0.3% in rinse-off products only as an active ingredient in functional cosmetics, and its use is prohibited in other products. This study aimed to develop of a HPLC method for the determination of TCS in cosmetics. The method was validated according to key parameters, including accuracy, precision, linearity, limit of detection (LOD), and limit of quantification (LOQ) in compliance with  the ICH guidelines and AOAC guidelines (Appendix K). The validation results showed excellent linearity, accuracy, precision and applicability. The validated methods were practically applied to 20 products were randomly selected including foam cleanser, shampoo, and body wash. As a result of the analysis, TCS was detected at a concentration of 0.26% in one product that listed TCS in its ingredient labeling. This concentration was in compliance with the domestic cosmetic regulations. The applicability assessment demonstrated that this method can be successfully applied to the rapid determination of TCS in cosmetic products, thereby contributing to the quality control.​