2025년
한국질량분석학회 여름학술대회 및 총회
2025. 08.27 (수) ~ 2025. 08.29 (금)
부산항국제전시컨벤션센터(BPEX)
초록검색
| 제목 | Authentic standards and matrix-based single analytical platform for targeted metabolomic analysis of 1,044 metabolites |
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| 작성자 | 강민식 (KIST) |
| 발표구분 | 포스터발표 |
| 발표분야 | 6. General |
| 발표자 |
강민식 (KIST) |
| 주저자 | 강민식 (KIST) |
| 교신저자 |
이재익 (KIST) |
| 저자 |
강민식 (KIST) 이민규 (KIST) 이지현 (KIST) 차상원 (동국대학교) 오한빈 (서강대학교) 이상규 (성균관대학교) 황금숙 (한국기초과학지원연구원) 이재익 (KIST) |
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Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-MS/MS) has become the de facto standard platform for metabolite identification and quantification in metabolomics. For successful metabolomic assessment studies, it is essential to detect, identify, and quantify a wide range of metabolites with high sensitivity and reproducibility. Furthermore, although recent advances in targeted metabolomics have enabled the profiling of hundreds of known metabolites, metabolite coverage is currently limited due to the restricted availability of authentic standards.
Here, we present an authentic standard and matrix-based analytical
method capable of simultaneously detecting and identifying over 1,000
metabolites from various human matrices in a single analytical run. To expand
metabolite coverage beyond commercially available standards, 302 MRM
transitions were obtained directly from biological matrices based on
characteristic MS/MS fragmentation patterns. This approach enabled the
inclusion of diverse metabolite classes, including sphingolipids,
glycerophospholipids, acylcarnitines, N-acyl taurines, and acyl CoAs. The present authentic
standard- and matrix-based method offers broad metabolite coverage, high
sensitivity, and excellent chromatographic behavior, demonstrating significant
potential for advancing large-scale targeted metabolomics.
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