2025년
한국질량분석학회 여름학술대회 및 총회
2025. 08.27 (수) ~ 2025. 08.29 (금)
부산항국제전시컨벤션센터(BPEX)
초록검색
| 제목 | GC-MS/MS-Based Metabolic Profiling of Kidney and Liver in Calorie-Restricted Aging Rats |
|---|---|
| 작성자 | 최병찬 (순천대학교 약학대학) |
| 발표구분 | 포스터발표 |
| 발표분야 | 4. Medical / Pharmaceutical Science |
| 발표자 |
Byeongchan Choi (College of Pharmacy, Sunchon National University) |
| 주저자 | Byeongchan Choi (College of Pharmacy, Sunchon National University) |
| 교신저자 |
Man-Jeong Paik (College of Pharmacy, Sunchon National University, Suncheon 57922, Republic of Korea) |
| 저자 |
Byeongchan Choi (College of Pharmacy, Sunchon National University) Moongi Ji (College of Pharmacy, Sunchon National University) Jaeyeop Lim (College of Pharmacy, Sunchon National University) Hansol Kim (College of Pharmacy, Sunchon National University, Suncheon 57922, Republic of Korea) Huyn Woo Kim (Molecular Inflammation Research Center for Aging Intervention (MRCA), Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea) Hae Young Chung (Molecular Inflammation Research Center for Aging Intervention (MRCA), Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea) Man-Jeong Paik (College of Pharmacy, Sunchon National University, Suncheon 57922, Republic of Korea) |
|
Aging leads to deterioration of liver and kidney functions, yet the metabolic changes in these organs and the effects of calorie restriction (CR) remain incompletely understood. In this study, we used gas chromatography-tandem mass spectrometry (GC-MS/MS) to profile organic acids, free fatty acids, and amino acids in the liver and kidney of young (8 months, Y), old (23 months, O), and old calorie-restricted (23 months, OCR) rats. Method optimization enabled reliable quantification of 48 and 45 metabolites in the kidney and liver, respectively. In the kidney, malic acid levels were significantly elevated in the O group compared to Y, while 3-hydroxybutyric acid was significantly increased in the OCR group relative to O. In the liver, phenylacetic acid, valine, isoleucine, and tyrosine showed significant changes between Y and O groups, and 3-hydroxybutyric acid was upregulated in OCR versus O. These findings suggest aging is associated with mitochondrial dysfunction, impaired phenylalanine metabolism, and renal decline, whereas CR may attenuate renal aging by reducing oxidative stress, inflammation, and lipid accumulation, and enhance hepatic energy metabolism and antioxidant defense. Overall, this study provides insights into age-related metabolic alterations in the liver and kidney and highlights potential mechanisms by which CR mitigates aging effects in these organs. |
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