2025년
한국질량분석학회 여름학술대회 및 총회
2025. 08.27 (수) ~ 2025. 08.29 (금)
부산항국제전시컨벤션센터(BPEX)
초록검색
| 제목 | Metabolomic Analysis of Urinary Nucleosides in Rats Following Intraperitoneal Administration of γ-Hydroxybutyric Acid Using LC-MS/MS |
|---|---|
| 작성자 | 최병찬 (순천대학교 약학대학) |
| 발표구분 | 포스터발표 |
| 발표분야 | 4. Medical / Pharmaceutical Science |
| 발표자 |
Byeongchan Choi (College of Pharmacy, Sunchon National University) |
| 주저자 | Byeongchan Choi (College of Pharmacy, Sunchon National University) |
| 교신저자 |
Man-Jeong Paik (College of Pharmacy, Sunchon National University) |
| 저자 |
Byeongchan Choi (College of Pharmacy, Sunchon National University) Moongi Ji (College of Pharmacy, Sunchon National University) Jiyeon Park (College of Pharmacy, Sunchon National University) Hyeon-Seong Lee (Korea Institute of Science and Technology, Gangneung Institute of Natural Products, Gangneung 25451, Republic of Korea) Chan Seo (Division of Natural Products Research, Honam National Institute of Biological Resource, Mokpo 58762, Republic of Korea) Meejung Park (4National Forensic Service, 10 Ipchoon ro, Wonju, Kangwon do 220 170, Republic of Korea) Sooyeun Lee (College of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Dalseo-gu, Daegu 704-701, Republic of Korea) Man-Jeong Paik (College of Pharmacy, Sunchon National University) |
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γ-Hydroxybutyric acid (GHB) is a central nervous system depressant with high addiction potential, known to induce oxidative stress and neurotoxicity. However, the underlying biochemical mechanisms remain unclear. Notably, no previous metabolomics study has investigated urinary nucleoside profiles and pattern changes following intraperitoneal GHB administration in rats.This study aimed to investigate changes in urinary nucleoside profiles following intraperitoneal GHB administration in rats. Ten nucleosides were quantified in control, single-dose, and multiple-dose GHB groups. Most nucleoside levels, excluding adenosine and 5'-deoxy-5'-methylthioadenosine (MTA), were higher in the single-dose group than in the multiple-dose group. Notably, adenosine levels increased by 421% and 729% in the single and multiple administration groups, respectively. Multivariate analyses (PCA and PLS-DA) identified six key nucleosides—cytidine, pseudouridine, uridine, N4-acetylcytidine, adenosine, and MTA—as major contributors to group separation. ROC analysis further supported their classification potential, distinguishing control from GHB-treated group. These findings indicate that urinary nucleoside metabolic patterns are altered following GHB administration. Cytidine, pseudouridine, uridine, N4-acetylcytidine, adenosine, and MTA are proposed as potential urinary biomarkers of GHB exposure and addiction. |
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