백주희 (충남대학교 분석과학기술대학원)

안현주 (충남대학교 분석과학기술대학원)

백주희 (충남대학교 분석과학기술대학원)

정혜경 (충남대학교 분석과학기술대학원)

오명진 (충남대학교 분석과학기술대학원)

안현주 (충남대학교 분석과학기술대학원)

The extracellular matrix(ECM) is a key structural component that mediates cell-cell interactions, maintains tissue architecture, and regulates signaling pathways. ECM proteins are essential for physiological homeostasis and are also involved in various pathological conditions, including inflammation, cancer, and neurodegeneration. Recent studies have highlighted the potential of ECM proteins, which represent essential components of the extracellular environment, as diagnostic biomarkers and therapeutic targets in various diseases. However, efficient isolation and analysis of ECM proteins remains a significant challenge, resulting in a limited number of studies and databases. In this study, we applied a chemical decellularization approach using representative detergents, including Triton X-100 to isolate ECM components from mouse kidney and brain tissues. The collected ECM protein samples were analyzed qualitatively and quantitatively using high-sensitivity, high-resolution Orbitrap-based nanoLC-MS/MS. This analytical strategy enabled the identification of tissue-specific ECM proteins and precise comparison of their relative abundance patterns. As a result, Collagen and Laminin were identified in both kidney and brain, but the overall distribution of ECM proteins differed between the two tissues. These findings support the future development of optimized, tissue-specific decellularization protocols aimed at preserving the native ECM protein composition of each organ.