김나경 (서강대학교 )

오한빈 (서강대학교)

김나경 (서강대학교 )

오한빈 (서강대학교)

​This study presents the development of a web-based backend software for controlling a custom-built automated sample preparation system for mass spectrometry. The system was previously developed in-house using open-source 3D printer technology, incorporating both open hardware and software. While commercial instruments are often constrained by closed-source designs, the software developed in this study is released as open-source, enabling flexible customization for specific experimental needs and offering a cost-effective, precise, and adaptable platform for protocol development.

The backend software comprises four microservices: Hardware Controller, Method Compiler, Database, and Security. The Hardware Controller runs on a Linux-based microcomputer (Raspberry Pi) using Klipper and Moonraker—open-source tools originally developed for 3D printer control—enabling precise motion control of the automated sample preparation system. The Method Compiler parses procedural text using natural language processing (NLP) tools and converts it into G-code, a standard machine instruction set that drives the coordinated movement of the automation system. The Database stores reagent information such as positions and volumes, experimental protocols and results, and logs all events that occur during experiments. The Security service controls user access in multi-user environments and enforces system-wide protection through authentication, authorization, and intrusion prevention.