노종성 (한국과학기술연구원)

최만호 (한국과학기술연구원)

노종성 (한국과학기술연구원)

최만호 (한국과학기술연구원)

Cortisol and 11-oxygenated androgens are closely associated with various adrenal diseases, and accurate quantification of these steroids is essential for clinical interpretation.  In this study, 2-hydrazinopyridine (2-HP) was employed to derivatize the carbonyl groups of nine 17α-hydroxysteroids involved in cortisol biosynthesis and four 11-oxygenated androgens.  The derivatization reaction was optimized by evaluating temperatures of 40, 60, and 80 °C, and reaction times of 20, 30, and 60 min.  The optimal condition was determined to be 60 °C for 20 min using 50 μL of 100 m<i style="font-family: "Times New Roman", serif; font-size: 12pt; text-indent: 12pt;">M</i> 2-HP in methanol.  Among the 17α-hydroxysteroids, analytical sensitivity for 17α-hydroxypregnenolone, cortisol, cortisone, 11-deoxycortisol, 6β-hydroxycortisol, allo-tetrahydrocortisol, and tetrahydrocortisone increased by up to 4-fold.  For 11-oxygenated androgens, including 11β-hydroxyandrostenedione (11β-OHA4), 11-ketoandrostenedione (11-KA4), and 11-ketotestosterone (11-KT), detectability improved by 1.4- to 2.6-fold, except for 11-KT.  Given the pivotal role of cortisol metabolism in adrenal physiology and the adrenal-specific nature of 11-oxygenated androgens, enhancing their detectability through 2-HP derivatization supports its clinical utility for precise and reliable steroid profiling in adrenal function assessment.