여름정기학술대회
2022여름초록
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포스터발표 |
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Brief Oral Presentation 발표신청 | |
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국가 |
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공동저자
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접수자
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Oxycholesterols, oxidized cholesterol metabolites, are important roles for regulator of hepatic
cholesterol and stimulation of reverse cholesterol transport. These metabolites also contribute to
accelerating the progression of inflammatory and fibrogenic response in the liver. Therefore,
oxycholesterols are associated with a broad range of metabolic disorders such as non-alcoholic
steatohepatitis (NASH). For these reasons, oxycholesterols are interesting candidates as potential
biomarkers of NASH. In this study, we developed analytical method and simultaneously analyzed
six OHCs (24S-, 25-, 27-OHCs and 24S-, 25-, 27-diOHCs) levels in human liver microsomes. The
profiles of OHCs were also performed in various animals such as dog, rat and mouse liver
microsomes by LC-ESI/MS/MS with picolinyl ester (PE) derivatization. In addition, we
investigated endogenous levels of OHCs in pooled normal and NASH human liver microsomes.
As results, endogenous levels of 24-OHC and 27-OHC were obtained in range of 0.531 to 0.559
ng/mL and 2.652 to 2.905 ng/mL for in normal liver microsomes, and were obtained in range of
0.625 to 0.703 ng/mL and 2.269 to 2.450 ng/mL for NASH liver microsomes, respectively. The
OHCs profiling showed that 24-OHC was increased and 27-OHC was reduced in NASH liver
microsomes, compared with controls (p<0.01).
Therefore, we presented the potential of LC-ESI/MS/MS with PE derivatization method and
applied for simultaneous quantification of six OHCs in liver microsomes. The present method is
clinically suitable for diagnosis.
* This research was supported by the Basic Science Research Program through the National
Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.
2020R1F1A1071199).
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